NIR Laser Photobiomodulation Induces Neuroprotection in an Vitro Model of Cerebral Hypoxia/Ischemia

NIR Laser Photobiomodulation Induces Neuroprotection in an Vitro Model of Cerebral Hypoxia/Ischemia

Introduction: The capability of PBM for ischemic stroke needs to be further explored and its mechanisms of action remain currently unclear. The aim of the present research was to identify a treatment protocol capable of inducing neuroprotection and to investigate the molecular mechanisms activated by a dual-wavelength near infrared (NIR) laser source in an organotypic hippocampal slice model of hypoxia/ischemia. Hippocampal slices were exposed to oxygen and glucose deprivation (OGD) for 30 min followed by NIR laser light (fluence 3.71, 7.42, or 14.84 J/cm2; wavelengths 808 nm and 905 nm) delivered immediately or 30 min or 60 min after OGD, in order to establish a therapeutic window.

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